dna damage repair markers  (Proteintech)

Journal: Cells

Article Title: Chronic Low-Dose-Rate Radiation-Induced Persistent DNA Damage and miRNA/mRNA Expression Changes in Mouse Hippocampus and Blood

doi: 10.3390/cells13201705

Figure Lengend Snippet: Immunohistochemical staining of the dentate gyrus of the hippocampus in the control and experiment mice. ( A1 – A3 ): NeuN immunopositive mature neurons (arrow); ( B1 – B3 ): GFAP immunopositive astrocytes (arrow); ( C1 – C3 ): PDGFRα immunopositive oligodendrocyte precursor cells (arrow) in the hilus; ( D1 – D3 ): IBA1 immunopositive microglia (arrow) in the hilus and the granule cell layer; ( E1 – E3 ): DCX immunopositive immature neurons (arrow) in the subgranular zone; ( F1 – F3 ): γH2AX immunostaining shows DNA damage foci in the granule cells. Scale bar = 100 μm in ( A1 ) applies to ( B1 – E1 ) and ( A2 – E2 ). Scale bar = 50 μm in ( F1 ) applies to ( F2 ). ( A3 – F3 ): statistical results. * p < 0.05. Control: n = 8; exp group: n = 7.

Article Snippet: Six antibodies were used in immunostaining, namely newly generated neuronal marker doublecortin (DCX, 1:500, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), mature neuronal marker NeuN (1:1000, Invitrogen, MA, USA), oligodendrocyte precursor cell marker platelet-derived growth factor receptor alpha (PDGFRα, 1:200, Cell Signaling Technology, Danvers, MA, USA), astrocyte marker glial fibrillary acidic protein (GFAP, 1:200, Cell Signaling Technology, Danvers, MA, USA), microglial marker ionized calcium-binding adapter molecule 1 (IBA1, 1:200, Cell Signaling Technology, Danvers, MA, USA), and DNA damage and repair marker γH2A histone family member X (γH2AX,1:200, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Immunohistochemical staining, Staining, Control, Immunostaining